MEKC Technique-Based Dairy Product Biochemical Analysis
RESEARCH ARTICLE

MEKC Technique-Based Dairy Product Biochemical Analysis

Wei Wang, * Open Modal
Authors Info & Affiliations
The Open Biotechnology Journal 12 Nov 2015 RESEARCH ARTICLE DOI: 10.2174/1874070701509010258

Abstract

The paper applies MEKC technique to simultaneously carry out quantitative analysis on dairy product’s melamine (Mel) and 5-hydroxymethyl furfural (HMF) bi-component. The quantization analytic experiment starts with a systematic research on operating voltage, electrophoretic buffer solution gravity and optimum pH value, which further influences additives’ concentration as well as other factors, and detects 10 kinds of practical samples on the basis of defined optimum conditions. Detection result indicates that both Mel and HMF showed peaks in 16min, simultaneously acquiring two indicator parameters in one analytical test, This test verifies applicability and scientific validity of MEKC technical approach in dairy product’s Mel and HMF contents detection, which can play an effective role in providing rapid and reliable daily monitoring support for dairy industry.

Keywords: 5-hydroxymethyl furfural, capillary electrophoresis, dairy product, melamine, standard extract.