Genetic Modification of MEOR Bacterium Bacillus licheniformis H Strain by Low Energy Ion Beam Irradiation
RESEARCH ARTICLE

Genetic Modification of MEOR Bacterium Bacillus licheniformis H Strain by Low Energy Ion Beam Irradiation

The Open Biotechnology Journal 26 Mar 2010 RESEARCH ARTICLE DOI: 10.2174/1874070701004010014

Abstract

The microbial enhanced oil recovery (MEOR) related microorganism, identified as Bacillus licheniformis by physiological and biochemical analyses and 16S rDNA sequence analyses, was irradiated with 15keV N+ at the dose of 0~4×1015ions/cm2. The “saddle model” survival curve showed that the turning point is the dose of 2.5×1015ions/cm2, with the survival rate of 23.3% and the positive mutation rate of 36.7%. The single clone fermentation experiments show that with the rising of dose treated, the surface tension of the fermentation broth of each group had the tendency to decrease, while the group treated at the dose of 2.5×1015ions/cm2 had the lowest average surface tension. Ultimately five mutants were screened from the colonies and two trains, M314 and M325 with a stable expression after the evaluation of five generations were screened out and ready for next analysis. TLC and FT-IR of fermentation broth components showed that the effective component was cyclic lipopeptide. These research results laid a basis for further study in low energy ion beam induced mutation breeding of petroleum microbes.

Keywords: Petroleum microorganism, Ion Irradiation, Mutation breeding, Biosurfactant, MEOR.