RESEARCH ARTICLE


Genetic Modification of MEOR Bacterium Bacillus licheniformis H Strain by Low Energy Ion Beam Irradiation



Zhang Xiangshenga, b, Li Miaoc, Xiang Tingsheng*, b
a State Key Lab of Microbial Technology, Shandong University, Shandanan Road, Jinan 250100, P. R. China
b Key Laboratory of Exploration Technologies for Oil and Gas Resources, Ministry of Education, Yangtze University,Hubei Jingzhou, 434025, P. R. China
c Anhui Agri-Food Safe Key Lab, Anhui Agricultural University, Hefei, 230036, P. R. China


Article Metrics

CrossRef Citations:
0
Total Statistics:

Full-Text HTML Views: 211
Abstract HTML Views: 821
PDF Downloads: 280
Total Views/Downloads: 1312
Unique Statistics:

Full-Text HTML Views: 177
Abstract HTML Views: 549
PDF Downloads: 186
Total Views/Downloads: 912



© 2010 Xiangsheng et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Key Laboratory of Exploration Technologies for Oil and Gas Resources, Ministry of Education, Yangtze University,Hubei Jingzhou, 434025, P. R. China; Tel: +86-531-88362416; E-mail: xiangshengzh@hotmail.com


Abstract

The microbial enhanced oil recovery (MEOR) related microorganism, identified as Bacillus licheniformis by physiological and biochemical analyses and 16S rDNA sequence analyses, was irradiated with 15keV N+ at the dose of 0~4×1015ions/cm2. The “saddle model” survival curve showed that the turning point is the dose of 2.5×1015ions/cm2, with the survival rate of 23.3% and the positive mutation rate of 36.7%. The single clone fermentation experiments show that with the rising of dose treated, the surface tension of the fermentation broth of each group had the tendency to decrease, while the group treated at the dose of 2.5×1015ions/cm2 had the lowest average surface tension. Ultimately five mutants were screened from the colonies and two trains, M314 and M325 with a stable expression after the evaluation of five generations were screened out and ready for next analysis. TLC and FT-IR of fermentation broth components showed that the effective component was cyclic lipopeptide. These research results laid a basis for further study in low energy ion beam induced mutation breeding of petroleum microbes.

Keywords: Petroleum microorganism, Ion Irradiation, Mutation breeding, Biosurfactant, MEOR.