Measurement of the Bio-Mechanical Properties of Two Different Feeder Layer Cells
S. Romanazzo1, 2, 3, K. Uesugi4, 5, 7, A. Taniguchi1, 2, *, G. Forte6, K. Morishima5, 7
Identifiers and Pagination:Year: 2021
First Page: 12
Last Page: 18
Publisher Id: TOBIOTJ-15-12
Article History:Received Date: 7/5/2020
Revision Received Date: 6/10/2020
Acceptance Date: 7/10/2020
Electronic publication date: 12/02/2021
Collection year: 2021
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
We here present our findings on 2 types of feeder layers, one composed of mouse embryonic fibroblasts (MEF) and the second one of mouse skeletal myoblasts (C2Cl2) feeder cells.
The 2 feeder layers present a dramatic variance of intrinsic stiffness (142.68 ± 17.21 KPa and 45.78 ± 9.81 KPa, respectively).
Results and Conclusion:
This information could be used for a better understanding of cells and cell microenvironment mechano-physical characteristics that are influencing stem cell commitment, in order to develop a suitable engineered tissue for cardiac and skeletal muscle repair and a bio-actuator.