RESEARCH ARTICLE

Measurement of the Bio-Mechanical Properties of Two Different Feeder Layer Cells

The Open Biotechnology Journal 12 Feb 2021 RESEARCH ARTICLE DOI: 10.2174/1874070702115010012

Abstract

Introduction:

We here present our findings on 2 types of feeder layers, one composed of mouse embryonic fibroblasts (MEF) and the second one of mouse skeletal myoblasts (C2Cl2) feeder cells.

Methods:

The 2 feeder layers present a dramatic variance of intrinsic stiffness (142.68 ± 17.21 KPa and 45.78 ± 9.81 KPa, respectively).

Results and Conclusion:

This information could be used for a better understanding of cells and cell microenvironment mechano-physical characteristics that are influencing stem cell commitment, in order to develop a suitable engineered tissue for cardiac and skeletal muscle repair and a bio-actuator.

Keywords: Bio-mechanical properties, Cell sheet, Mouse embryonic fibroblasts (MEF), Mouse skeletal myoblast, Skeletal tensile test, Bio-actuator.
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