RESEARCH ARTICLE


Measurement of the Bio-Mechanical Properties of Two Different Feeder Layer Cells



S. Romanazzo1, 2, 3, K. Uesugi4, 5, 7, A. Taniguchi1, 2, *, G. Forte6, K. Morishima5, 7
1 Graduate School of Advanced Science and Engineering, Waseda University, Tokyo, Japan
2 Cell-Materials Interaction Group, Biomaterials Unit, International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS), Tsukuba, Japan
3 School of Chemistry, University of New South Wales (UNSW), Sydney, Australia
4 Graduate School of Mechanical Engineering, Department of Mechanical Engineering, Osaka University, Yamadaoka, Suita, Osaka, 565-0871, Japan
5 Department of Mechanical Systems Engineering, Ibaraki University, 4-12-1 Nakanarusawacho, Hitachi, Ibaraki, 316-8511, Japan
6 Center for Translational Medicine (CTM), International Clinical Research Center (ICRC) of St. Anne's University Hospital, Brno, Czech Republic
7 The Center for Advanced Medical Engineering and Informatics, Osaka University, Yamadaoka, Suita, Japan


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Creative Commons License
© 2021 Romanazzo et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Cell-Materials Interaction Group, Biomaterials Unit, International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS), Tsukuba, Japan; Tel: 0000-0001-7835-2522; E-mail: taniguchi.akiyoshi@nims.go.jp


Abstract

Introduction:

We here present our findings on 2 types of feeder layers, one composed of mouse embryonic fibroblasts (MEF) and the second one of mouse skeletal myoblasts (C2Cl2) feeder cells.

Methods:

The 2 feeder layers present a dramatic variance of intrinsic stiffness (142.68 ± 17.21 KPa and 45.78 ± 9.81 KPa, respectively).

Results and Conclusion:

This information could be used for a better understanding of cells and cell microenvironment mechano-physical characteristics that are influencing stem cell commitment, in order to develop a suitable engineered tissue for cardiac and skeletal muscle repair and a bio-actuator.

Keywords: Bio-mechanical properties, Cell sheet, Mouse embryonic fibroblasts (MEF), Mouse skeletal myoblast, Skeletal tensile test, Bio-actuator.