RESEARCH ARTICLE
Enhanced Interferon-α2b Production in Periplasmic Space of Escherichia coli through Medium Optimization using Response Surface Method
Joo Shun Tan1, Ramakrishnan Nagasundara Ramanan1, Siti Nor Ani Azaman1, Tau Chuan Ling2, Mustapha Shuhaimi3, Arbakariya B. Ariff*, 1, 4
Article Information
Identifiers and Pagination:
Year: 2009Volume: 3
First Page: 117
Last Page: 124
Publisher ID: TOBIOTJ-3-117
DOI: 10.2174/1874070700903010117
Article History:
Received Date: 22/05/2009Revision Received Date: 31/07/2009
Acceptance Date: 30/08/2009
Electronic publication date: 22/12/2009
Collection year: 2009
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
The influence of different carbon and nitrogen sources on growth of recombinant Escherichia coli and human interferon-α2b (IFN-α2b) production in periplasmic space was studied in shake flask culture. A statistical method based on Plackett-Burman design was used to screen the main medium components that greatly influenced the performance of the fermentation process. The optimization of medium was performed using response surface methodology (RSM) where three critical factors (glucose, yeast extract and peptone) were optimized using central composite design. The highest yield of periplasmic recombinant human interferon-α2b (PrIFN-α2b) (335.8 μg/L) was predicted to be obtained in optimized medium containing 5.47 g/L glucose, 55.24 g/L yeast extract and 42.27 g/L peptone.. The production of IFN-α2b in periplasmic space in optimized medium was about 2.5, 11.7 and 124.4 times higher than Terrific broth (TB), Luria-Bertani (LB), and minimal medium (M9), respectively.