REVIEW ARTICLE
The Effect of Serum on the PEG-Based Crosslinker-Induced Spheroid Formation of Pancreatic β-Cell
Michiko Ito, Tetsushi Taguchi*
Article Information
Identifiers and Pagination:
Year: 2008Volume: 2
First Page: 138
Last Page: 142
Publisher ID: TOBIOTJ-2-138
DOI: 10.2174/1874070700802010138
Article History:
Received Date: 17/12/2007Acceptance Date: 17/12/2007
Electronic publication date: 27/5/2008
Collection year: 2008
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
A polymeric crosslinker poly(ethylene glycol) derivative with dioleyl groups as hydrophobic group at both ends was developed for promoting cellular spheroid formation. Our approach to bridge cells was based on the crosslinking of cell membrane using a crosslinker via the hydrophobic interaction. Using the crosslinker, spheroid formation of pancreatic β-cell line RIN in a round bottom 96-well plate was evaluated, especially in effect of serum on spheroid formation. In the presence or absence of serum, the size of prepared spheroid was found to decrease with increasing culture time and with increasing crosslinker concentration. However, the medium without serum proved to be a favorable condition for promoting cell aggregation because in this case spheroid with smaller size could be obtained. It was clarified that spheroid formation and insulin secretion of the spheroid prepared by the crosslinker were enhanced, especially in the medium without serum.