REVIEW ARTICLE

Hydroxyapatite- and Amelogenin Protein-Induced Expression of Mineralization-Related Genes in a Dental Epithelial Cell Line

The Open Biotechnology Journal 27 May 2008 REVIEW ARTICLE DOI: 10.2174/1874070700802010116

Abstract

Hydroxyapatite (HAp), a major inorganic component of hard tissues, has been widely used as a novel scaffold for bone or tooth tissue regeneration. However, the effects of HAp on dental cells at the molecular level are poorly understood. In the present study, we evaluated the effects of HAp on differentiation- and mineralization-related gene expression in the dental epithelial cell line (HAT-7). HAT-7 cells were observed to spread on the surface of HAp sintered disks and to increase the expression of several differentiation- and mineralization-related genes. Furthermore, amelogenin and HAp synergistically induced differentiation resulting in increased amelogenin mRNA expression in HAT-7 cells. The results from this study provide important information to develop novel biomaterials for enamel regeneration.

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