RESEARCH ARTICLE

Recombinant Production and Molecular Docking Studies of Casoplatelin, a Bioactive Peptide

The Open Biotechnology Journal 19 Aug 2020 RESEARCH ARTICLE DOI: 10.2174/1874070702014010084

Abstract

Background:

Bioactive peptides from κ-casein have immense therapeutic potential as prophylactic formulations. Among these, casoplatelin is a κ-casein derived bioactive peptide with anti-thrombotic activities.

Aim:

Herein, we report the production of casoplatelin in an E. coli expression system (using a pBAD vector) and show in silico modeling of its interactions.

Methods:

A synthetic DNA construct encoding casoplatelin was designed with pepsin cleavage sites before and after the synthetic construct to allow the release of the peptide from the pro-peptide.

Results:

A novel recombinant approach was demonstrated for the production of casoplatelin, and anti-platelet aggregation activities of the product were confirmed. Also, casoplatelin structures were characterized in silico and then implemented to determine potential structural interactions with fibrinogen.

Conclusion:

The present study showcases the recombinant approach for biopeptide production and its interaction with fibrinogen through in silico approach.

Keywords: Casoplatelin, Bioactive peptide, Cloning, Molecular docking, Anti-thrombotic activity, Fibrinogen.
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