RESEARCH ARTICLE


Enhancement of Recombinant Antibody Expression Level by Growth Controlled Medium



Le Thi Minh Phuc1, 2, 3, Tetsuji Sasaki4, Hisayo Shimizu4, Nguyen Thi Minh Huyen3, Nguyen Thi Thu Thuy3, Le Quang Huan3, Akiyoshi Taniguchi1, 2, *
1 Cellular Functional Nanomaterials Group, Research Center for Functional Materials, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan
2 Graduate School of Advanced Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan
3 Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam
4 Kyokuto Pharmaceutical Industrial Co., Ltd. 3333-26 Aza-Asayama, Kamitezuna, Takahagi-shi, Ibaraki 318-0004, Japan


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© 2018 Phuc et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Cellular Functional Nanomaterials Group, Research Center for Functional Materials, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan; Tel: 81-29-860-4505; E-mail: taniguchi.akiyoshi@nims.go.jp


Abstract

Background:

Transient expression system is very widely used for protein expression including recombinant protein expression. Normally, in the commercially available expression system, cells were grown rapidly in protein expression. However, over cell growth was not only unnecessary for high level protein expression, but also induced cell death and led to protein degradation.

Aims and Objectives:

To overcome these limitations a new adapted culture method needs to be developed.

Methods:

In this work, we developed growth control medium for transient high protein expression system using NPLAd (Non-Protein and Lipid Medium Adopted) cells. With this system, cell numbers were not increased until 10 days.

Results:

Our results indicated that expression level in NPLAd system was 1.8 times higher than that in Free style system, which was one of the commercially available high expression systems.

Conclusion:

The antibody performance expressed by NPLAd system was almost the same to that of expressed by Free style system. The results suggested that NPLAd system could be useful for protein expression, such as recombinant antibody.

Keywords: Recombinant antibody, Protein expression, CHO cells, Culture medium, NPLAd system, Antibody performance.