Enhancement of Recombinant Antibody Expression Level by Growth Controlled Medium
Le Thi Minh Phuc1, 2, 3, Tetsuji Sasaki4, Hisayo Shimizu4, Nguyen Thi Minh Huyen3, Nguyen Thi Thu Thuy3, Le Quang Huan3, Akiyoshi Taniguchi1, 2, *
Identifiers and Pagination:Year: 2018
First Page: 78
Last Page: 85
Publisher Id: TOBIOTJ-12-78
Article History:Received Date: 16/2/2018
Revision Received Date: 6/4/2018
Acceptance Date: 10/4/2018
Electronic publication date: 23/05/2018
Collection year: 2018
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Transient expression system is very widely used for protein expression including recombinant protein expression. Normally, in the commercially available expression system, cells were grown rapidly in protein expression. However, over cell growth was not only unnecessary for high level protein expression, but also induced cell death and led to protein degradation.
Aims and Objectives:
To overcome these limitations a new adapted culture method needs to be developed.
In this work, we developed growth control medium for transient high protein expression system using NPLAd (Non-Protein and Lipid Medium Adopted) cells. With this system, cell numbers were not increased until 10 days.
Our results indicated that expression level in NPLAd system was 1.8 times higher than that in Free style system, which was one of the commercially available high expression systems.
The antibody performance expressed by NPLAd system was almost the same to that of expressed by Free style system. The results suggested that NPLAd system could be useful for protein expression, such as recombinant antibody.