RESEARCH ARTICLE


Detection and Molecular Characterization of Vibrio Parahaemolyticus in Shrimp Samples



Daryoush Asgarpoor1, 2, Fakhri Haghi2, Habib Zeighami 2, *
1 Student Research Center, Zanjan University of Medical Sciences, Zanjan, Iran
2 Department of Microbiology, Zanjan University of Medical Sciences, Zanjan, Iran


Article Metrics

CrossRef Citations:
0
Total Statistics:

Full-Text HTML Views: 759
Abstract HTML Views: 704
PDF Downloads: 580
ePub Downloads: 447
Total Views/Downloads: 2490
Unique Statistics:

Full-Text HTML Views: 429
Abstract HTML Views: 304
PDF Downloads: 254
ePub Downloads: 204
Total Views/Downloads: 1191



© 2018 Asgarpoor et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Department of Microbiology, Zanjan University of Medical Sciences, Zanjan, Iran, Tel: +982433140296, Fax: +982433449553; E-mail: zeighami@zums.ac.ir


Abstract

Background:

Food safety has emerged as an important global issue with international trade and public health implications. Bacterial pathogens asVibrio parahaemolyticus recognized as an important cause of foodborne diseases related to the consumption of raw, undercooked or mishandled seafood worldwide.

Methods:

A total of 70 individual wild shrimp samples were collected from shrimp retail outlets in Zanjan, Iran and investigated for the presence of potentially pathogenic strains of V. parahaemolyticus.The shrimp samples were immediately homogenized and cultured on TCBS agarand subjected to confirmatory biochemical tests. Polymerase Chain Reaction (PCR) was performed for detection of total and pathogenic V. parahaemolyticus by amplification of vp–toxR,tdh and trh genes.

Results:

The conventional method indicated that 16 (22.8%) of samples were positive for V. parahaemolyticus. However, PCR verified that only 12 (17.1%) shrimp samples were positive for V. parahaemolyticus. Of the 70 shrimp samples in our study, only 2 (2.8%) tdh and 1 (1.4%) trh positive strains were identified.

Conclusion:

Detection of tdh and/ or trh positive V. parahaemolyticus in shrimp marketed in Zanjan, Iran shows a probable risk for public health. Therefore, the reliable molecular methods for monitoring of potentially pathogenic V. parahaemolyticus are strongly recommended for the routine seafood examination.

Keywords: Vibrio parahaemolyticus, Shrimp, PCR, Molecular characterization, vp–toxR, tdh, trh genes.