RESEARCH ARTICLE

Ochratoxin A Removal by Lactobacillus Plantarum V22 in Synthetic Substrates

The Open Biotechnology Journal 28 December 2018 RESEARCH ARTICLE DOI: 10.2174/1874070701811140282

Abstract

Background:

Ochratoxin A is a nephrotoxin which may occur in wines characterised by higher pH than the average. In the last decades the mechanisms responsible for ochratoxin A reduction by lactic acid bacteria have been investigated and identified as mainly cell walls adsorption and / or enzymatic conversion to ochratoxin-α, a non-toxic metabolite. Since lactic acid bacteria are involved in the malolactic fermentation during the wine-making process, selected starter cultures could be exploited to guarantee safe ochratoxin A level in wines also from contaminated grapes. A lactic acid bacteria strain (Lactobacillus plantarum V22) was previously selected for its ability of both degrading ochratoxin A and carrying out malolactic fermentation at high pH.

Objective:

This study was aimed at assessing if the selected L. plantarum strain, can reduce ochratoxin A because it can use it as a carbon source.

Methods:

L. plantarum V22 was grown in the presence of ochratoxin A in two different synthetic substrates, with or without malic acid, monitoring the reduction of ochratoxin A and the presence of ochratoxin α as an indicator for a toxin enzymatic hydrolysis. The presence of residual not hydrolysed ochratoxin A bound to the bacteria cell walls was also evaluated to quantify the ochratoxin A removal due to simple adsorption.

Result:

A significant reduction of 19.5 ± 2.0% in ochratoxin A concentration was observed only in the presence of malic acid. The quantified fraction of ochratoxin A adsorbed on cell walls was irrelevant and the metabolite ochratoxin α could not be detected.

Conclusion:

There is a possibility that L. plantarum V22 can degrade ochratoxin A through a not yet identified metabolic pathway.

Keywords: Bio-decontamination, Lactobacillus plantarum, Malolactic fermentation, Ochratoxin A, Wine, Synthetic Substrates.
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