RESEARCH ARTICLE
An Easy Method for Screening and Detection of Laccase Activity
Albino A. Dias*, António J.S. Matos, Irene Fraga, Ana Sampaio, Rui M.F. Bezerra
Article Information
Identifiers and Pagination:
Year: 2017Volume: 11
First Page: 89
Last Page: 93
Publisher ID: TOBIOTJ-11-89
DOI: 10.2174/1874070701711010089
Article History:
Received Date: 14/03/2017Revision Received Date: 21/07/2017
Acceptance Date: 13/08/2017
Electronic publication date: 14/09/2017
Collection year: 2017
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Objective:
An instrument-free assay was developed for simultaneous detection of laccase activity in a large number of samples as diverse as screening of laccase-producing microbial cultures or chromatographic fractions.
Method:
Dried paper discs previously impregnated with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) were placed on a flat-bottom microplate (a simple way to avoid misidentification) and loaded with an aliquot from each sample.
Results:
Discs corresponding to samples containing laccase activity become green-bluish colored within first ten minutes of reaction, allowing direct detection through simple naked-eye inspection.
Conclusion:
As an example, this easy process was applied to the laccase purification in order to eliminate chromatographic fractions that did not contain laccase activity, thus reducing the number of spectrophotometric assays.