Evaluation of Fixed-Bed Cultures with Immobilized Lactococcus Lactis ssp. Lactis on Different Scales

Rebecca Faschian, Ilyas Eren, Steven Minden, Ralf Pörtner*
Hamburg University of Technology, Institute of Bioprocess and Biosystems Engineering, Denickestr, Hamburg, Germany

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© 2017 Faschian et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: ( This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

these authors contributed equally to this work* Address correspondence to this author at the Hamburg University of Technology, Institute of Bioprocess and Biosystems Engineering, Denickestr. 15, D-21073 Hamburg, Germany; Tel: +49 40 42878 2886; Fax: +49 40 42878 2909; E-mail:


Fixed-bed processes, where cells are immobilized within macroporous carriers, are a promising alternative to processes with suspended cells. A scale-up concept is presented in order to evaluate the performance as part of process design of fixed-bed processes. Therefore, Lactococcus lactis cultivation in chemostat and batch mode was compared to fixed bed cultures on three different scales, the smallest being the downscaled Multiferm with 10 mL fixed bed units, the second a 100 mL fixed-bed reactor and the third a pilot scale reactor with 1 L fixed bed volume. As expected, the volume specific lactate productivity of all cultivations was dependent on dilution rate. In suspension chemostat culture a maximum of 2.3 g·L-1·h-1 was reached. Due to cell retention in the fixed-beds, productivity increased up to 8.29 g·L-1·h-1 at a dilution rate of D = 1.16 h-1 (corresponding to 2.4·µmax) on pilot scale. For all fixed bed cultures a common spline was obtained indicating a good scale-up performance.

Keywords: Fixed-bed reactor, Immobilization, Lactococcus lactis, Scale-up.