RESEARCH ARTICLE


Transferability of Sorghum Microsatellite Markers to Bamboo and Detection of Polymorphic Markers



Tesfaye Disasa1, *, Tileye Feyissa2, Demissew Sertse3
1 National Agricultural Biotechnology Research Center, P.O. Box 2003, Addis Ababa, Ethiopia
2 Faculty of Life Sciences, Addis Ababa University, P.O. Box 1176, Addis Ababa, Ethiopia
3 University of Ottawa, K1Z7P2, 136 Crerar, Ottawa, ON, Canada


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© Disasa; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution-Non-Commercial 4.0 International Public License (CC BY-NC 4.0) (https://creativecommons.org/licenses/by-nc/4.0/legalcode), which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the National Agricultural Biotechnology Research Center, P.O. Box 2003, Addis Ababa, Ethiopia; Tel: +251 911118068; Fax: +251 112370377; E-mail: tesfayedisasa@yahoo.com


Abstract

The use of molecular markers for the characterization and evaluation of plant genetic resources has become a useful approach in plant genetic research. Simple Sequence Repeats (SSRs) are among the markers that are widely used in genetic diversity and parental analysis owing to their co-dominant nature, high reproducibility, abundance in the genome and transferability across species or genera. The development of these markers for a species might be costly and time consuming. Hence, screening existing markers through transferability test from closely related species or family is resource conscious. In this study, the transferability of 90 polymorphic SSR markers of sorghum to bamboo was tested and polymorphic analysis of transferable markers were performed. Nearly 62% of the tested SSRs successfully recorded amplification in at least one bamboo species of which 55% were polymorphic. These polymorphic markers detected a total of 147 alleles at an average rate of 4.7 alleles per marker. The abundant alleles account 20.4% while the common and rare alleles share 39.6 and 40 %, respectively. The result showed a relatively low degree of polymorphic information content (PIC) averaging 0.29. The gene diversity index (He) ranged from 0.21 to 0.49 with a mean of 0.37. The cluster analysis based on the polymorphic markers surfaced most of the species in accordance with their geographic origin. The complementarity of the weighted neighbour joining tree and coordinate analysis implies the representative nature of the transferred markers for the diversity analysis of bamboo species.

Keywords: Bamboo, genetic diversity, PCR analysis, polymorphic information content, sorghum, SSR markers, transferability.