RESEARCH ARTICLE

Multi-Fixed-Bed Bioreactor System Applied for Bioprocess Development of Immobilized Lactic Acid Bacteria

The Open Biotechnology Journal 19 Feb 2016 RESEARCH ARTICLE DOI: 10.2174/1874070701610010001

Abstract

The multi-fixed-bed bioreactor “Multiferm” provides a downscaled system for the development of fixed-bed processes with immobilized microorganisms or cells. It consists of twelve single fixed-bed units that can be operated independently. Different bacterial strains, carriers used for immobilization, media or flow rates can be studied in one compact experimental set-up. Experiments can be run in parallel to allow for determination of experimental errors. In these studies several continuous cultivations were carried out with Lactobacillus delbrueckii ssp. bulgaricus using different dilution rates and culture durations as proof-of-concept for the Multiferm. Parameters relevant for process design such as reproducibility of simultaneously performed cultures, optimal dilution rate and long-term performance were determined by means of volume specific lactate and biomass productivity. First indications on a reduced growth rate of the cells due to immobilization were found.

Keywords: Cell density, continuous cultivation, dilution rate, experimental error, fixed-bed, lactic acid bacteria, metabolic activity, process development, scale-down.
Fulltext HTML PDF ePub
1800
1801
1802
1803
1804