REVIEW ARTICLE


The Effect of Metal Materials on Heat Shock Protein 70B’ Gene Expression



Junko Okuda-Shimazaki1, Akiko Yamamoto2, Daisuke Kuroda3, Takao Hanawa4, Akiyoshi Taniguchi*, 1
1 Cell-Sensing Group, Biomaterials Center, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan
2 Biometal Group, Biomaterials Center, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan
3 Department of Materials Science and Engineering, Suzuka National College of Technology, Shiroko, Suzuka, Mie 510-0294, Japan
4 Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-surugadai, Chiyoda-ku, Tokyo 164-0011, Japan


Article Metrics

CrossRef Citations:
3
Total Statistics:

Full-Text HTML Views: 2082
Abstract HTML Views: 1906
PDF Downloads: 770
Total Views/Downloads: 4758
Unique Statistics:

Full-Text HTML Views: 964
Abstract HTML Views: 1097
PDF Downloads: 526
Total Views/Downloads: 2587



Creative Commons License
© 2008 Okuda-Shimazaki et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Cell-Sensing Group, Biomaterials Center, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan; Phone: +81-29-860-4505; Fax: +81-29-860-4714; E-mail: TANIGUCHI.Akiyoshi@nims.go.jp


Abstract

To avoid the toxic effect of released nickel ions and compounds from conventional stainless steels, nickel-free austenitic stainless steels have been developed. We previously established a new manufacturing process to produce nickel-free austenitic stainless steel that involves nitrogen adsorption treatment. Although the cytocompatibility of nickelfree austenitic stainless steel produced using this method has been evaluated using two viability assay, molecular level analysis, such as gene expression analysis, has not been previously performed. In the present study, the cytotoxicity of our nickel-free austenitic stainless steel, as well as of commercially available metal materials, was evaluated by analysis of heat shock protein 70B’ (HSP70B’) gene expression as a stress response marker. Furthermore, to investigate the effect of metal materials on cytotoxicity, HSP70B’ gene expression was quantified using human osteoblast-like SaOS-2 cells, human monocyte THP-1 cells and the mouse macrophage cell line J774A.1. We found no significant differences in HSP70B’ expression among the various metal materials, including the nickel-free austenitic stainless steel, indicating that the nickel-free austenitic stainless steel produced using our nitrogen adsorption method has the same cytocompatibility as commercially available metal materials.