RESEARCH ARTICLE

Responses of Germlings to Acute Environmental Stress

The Open Biotechnology Journal 10 Sept 2015 RESEARCH ARTICLE DOI: 10.2174/1874070701509010127

Abstract

The responses of Sargassum thunbergii germlings to high temperature, low salinity, desiccation, combined thermal and osmotic stress (35 ºC combined with 12 psu), anthracene, and eutrophication were examined. Probit regression analysis results showed that the median lethal time (LT50) values of high temperature decreased with the increase in temperature. The 24 h median lethal temperature was 36.9 ºC. For salinity treatment, the LT50 value of fresh water was 47.6 h. Survival rates of germlings were over 60% when germlings were exposed to salinities ranging from 27 psu to 7 psu at a time interval of 108 h post-treatment. The LT50 values of desiccation and combined thermal and osmotic stress (35 ºC combined with 12 psu) were 7.0 h and 9.8 h, respectively. Anovas showed that germlings were inhibited by high concentrations of anthracene (5 mg L-1 and 10 mg L-1) with low survival rates of below 50% and low relative growth rates of below 1% after 25 days of culture; however, low concentrations (0.01-1 mg L-1) had no significant effects. In addition, neither severe eutrophication nor disproportionality of N/P showed any significant effect on the survival and growth of germlings. Of the environmental stresses tested, possible occurrence of high temperature of 40 ºC and combined thermal and osmotic stress directly impacted the survival of germlings, suggesting that the deterioration of S. thunbergii bed may be related more to increasing extreme climatic events.

Keywords: Environmental stress, germling, median lethal value, Sargassum thunbergii.
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