Mating Types Analysis of Magnaporthe oryzae Populations by Molecular Methods
Soma Samanta, Urmila Dhua*, Shubhransu Nayak, L. Behera, A. K. Mukherjee
Identifiers and Pagination:Year: 2014
First Page: 6
Last Page: 12
Publisher ID: TOBIOTJ-8-6
Article History:Received Date: 05/08/2014
Revision Received Date: 08/09/2014
Acceptance Date: 15/09/2014
Electronic publication date: 9/12/2014
Collection year: 2014
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Rice blast is a devastating disease which is caused by the heterothallic fungus Magnaporthe oryzae. Compatible sexual recombination which occurs between two M. oryzae strains of different mating types, can enhance genetic variability. Assessment of mating type alleles is used as a marker to measure population diversity. Forty six isolates of M. oryzae were collected from infected rice leaves from various ecosystems of coastal Odisha, India, and the mating type analysis using molecular markers was carried out. MAT1-1 mating type was dominating in all the ecosystems and MAT1-2 was found to be present in uplands as well as in irrigated fields. Both mating types could be found in the same field in irrigated ecosystem. The disease spread was very fast vertically as well as horizontally in those fields resulting in blast lesions looking as ‘green islands (gi) produced in senescence leaves’, and MAT1-2 was found to be associated with all gi lesions. Consequently, the management of the disease in those plots was very difficult. Interestingly, ribosomal RNA IGS region could not be amplified in MAT1-2 isolates but consistent amplification was obtained in MAT1-1 mating type isolates.