Assessment of the Potential of Lactic Acid Bacteria as Dried Starter Culture for Cereal Fermentation
RESEARCH ARTICLE

Assessment of the Potential of Lactic Acid Bacteria as Dried Starter Culture for Cereal Fermentation

The Open Biotechnology Journal 21 Feb 2014 RESEARCH ARTICLE DOI: 10.2174/1874070701408010001

Abstract

The kinetic growth parameters [maximal specific growth rate μm (h-1), and generation time g (h)] and viability after stress treatments (dehydration with glycerol solution of increasing concentration, freezing or heating stress) of four lactic acid bacteria [Lactobacillus plantarum (LP), L. fermentum (LF), Leuconostoc spp. (M1) and Lactococcus spp. (M2)] were determined. The maximal specific growth rate (μm) and generation time g ranged between 0.51 h-1 to 0.14 h-1 and 0.61 h to 1.33 h, respectively. We observed that the strains were sensitive to a change in water activity (aw 0.32 final), storage and incubation temperatures. The Strain LF obtained a higher cell concentration and viability, as well as a lower g compared to those obtained by the other strains. The kinetic growth parameters (μm, g) together with viability after stress treatments could be used for the screening of dried lactic acid starter cultures.

Keywords: Dehydration tolerance, generation time, lactic acid bacteria, starter cultures, stress treatment, viability, water activity.