REVIEW ARTICLE
Screening of Xanthine Oxidase Producing Microorganisms Using Nitroblue Tetrazolium Based Colorimetric Assay Method
Amit Agarwal, U.C. Banerjee*
Article Information
Identifiers and Pagination:
Year: 2009Volume: 3
First Page: 46
Last Page: 49
Publisher ID: TOBIOTJ-3-46
DOI: 10.2174/1874070700903010046
Article History:
Received Date: 03/02/2009Revision Received Date: 02/04/2009
Acceptance Date: 29/04/2009
Electronic publication date: 21/5/2009
Collection year: 2009
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Xanthine oxidase is a highly versatile and ubiquitous complex molybdoflavoprotein, which controls the rate limiting step of purine catabolism pathway. Microbial xanthine oxidase can be used to address a number of questions presently not feasible with the eukaryotic enzymes. In the present study, a high-throughput microtitre plate-based colorimetric assay for xanthine oxidase producing microorganism was developed. Superoxides produced by microbial cultures, grown on xanthine rich medium interacts with nitroblue tetrazolium (NBT) solution and produces dark blue color, which facilitates the rapid screening of xanthine oxidase producing microorganisms and could be adapted for quick quantitative assessment and distribution of xanthine oxidase in many heterogeneous microbial communities. The method developed may be utilized for the rapid screening of a variety of xanthine oxidase producing microorganisms from the nature.