REVIEW ARTICLE


Enhancement of Extracellular Pullulanase Production by Raoultella planticola DSMZ 4617 Using Optimized Medium Based on Sago Starch



Siew Ling Hii1, #, Tau Chuan Ling2, Rosfarizan Mohamad1, 3, Arbakariya B. Ariff1, 3, *
1 Laboratory of Industrial Biotechnology, Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
2 Department of Process and Food Engineering, Faculty of Engineering, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
3 Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia and
# Present address: Department of Bioscience and Chemistry, Faculty of Engineering and Science, Universiti Tunku Abdul Rahman, 53300 Setapak, Kuala Lumpur, Malaysia


Article Metrics

CrossRef Citations:
8
Total Statistics:

Full-Text HTML Views: 1823
Abstract HTML Views: 2207
PDF Downloads: 862
Total Views/Downloads: 4892
Unique Statistics:

Full-Text HTML Views: 803
Abstract HTML Views: 1208
PDF Downloads: 609
Total Views/Downloads: 2620



Creative Commons License
© 2009 Hii et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; Tel: +60389467516; Fax: +603 89467510; E-mail: arbarif@biotech.upm.edu.my


Abstract

Enhancement of pullulanase production by Raoultella planticola DSMZ 4617 using optimized medium formulation was investigated in batch fermentation using 500-mL shake flask. The fermentations were carried out, firstly, to search for a suitable cultivation medium for enzyme production and followed by the evaluations on the influence of carbon and nitrogen sources and also initial culture pHs on the secretion of pullulanase by this bacterium. The modified mineral Czapek medium was found suitable to produce substantially high activity of pullulanase (320 times higher) as compared to ‘Ohba-Ueda’ medium. This bacterium was found superior in pullulanase production using sago starch and peptone as carbon and nitrogen sources, respectively. Using the optimized medium, the bacterium produced 0.95 U/mL of pullulanase at initial culture pH of 7 and incubation temperature of 30oC.

Keywords: Pullulanase, Raoultella planticola DSMZ 4617, submerged fermentation, sago starch.