REVIEW ARTICLE


Cytotoxic Activity of CD48 Monoclonal Antibodies Against Human Lymphoma Cells



Jiewei Wei1, David Y. Chin1, 2, Stephen M. Mahler1, 2, *
1 School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, Australia 2052 and
2 Current Address: Australian Institute for Bioengineering and Nanotechnology, University of Queensland, Brisbane, Queensland, Australia 4072


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Creative Commons License
© 2008 Wei et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Australian Institute for Bioengineering and Nanotechnology, University of Queensland, Brisbane, Queensland, Australia 4072; Tel: +61-7-33463175; Fax: +61-7-33463973; E-mail: s.mahler@uq.edu.au


Abstract

CD48 is a cell surface, glycosylphosphatidylinositol-linked glycoprotein, and a potential target for treatment of leukemia and lymphoma. Two anti-CD48 mAbs, murine HuLy-m3 and human IgG1-N2A, were compared in cellular assays using a human lymphoma cell line (Raji) for their ability to inhibit cell growth and induce apoptosis. In vitro studies revealed both HuLy-m3 and IgG1-N2A mAbs were able to induce potent growth inhibition, reflected by a reduction in viable cells of approximately 70% compared to controls after 90 h. Furthermore, Raji cells treated with IgG1-N2A showed evidence of apoptosis, including increased ethidium bromide uptake, cell shrinkage and chromosomal DNA degradation.