REVIEW ARTICLE
Ferrous Iron Oxidation by Immobilized on Refractory Clay Tiles
E.R. Donati*
Article Information
Identifiers and Pagination:
Year: 2008Volume: 2
First Page: 190
Last Page: 194
Publisher ID: TOBIOTJ-2-190
DOI: 10.2174/1874070700802010190
Article History:
Received Date: 17/03/2007Revision Received Date: 23/06/2008
Acceptance Date: 25/06/2008
Electronic publication date: 4/7/2008
Collection year: 2008
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Refractory clay tiles are composed of kaolin, which is the commercial name of clay, and this consists mainly of kaolinite mineral. Two such tiles and caowool packed in glass columns were used for the immobilization of Acidithiobacillus ferrooxidans cells in a ferrous iron medium, which was percolated through the supports. Colonization was carried out by several media replacements with no further inoculation until maximum ferric iron productivity was reached. One of the tiles was discarded due to the high iron precipitation during bacterial growth. The columns with the other supports were used for ferrous iron oxidation in batch and continuous flow modes of operation and these appeared to be promising supports for A. ferrooxidans. A ferrous iron oxidation rate of 14.5 mmol.l-1.h-1 was reached in one of the columns in the continuous culture. After being used for several cultures, pieces of tiles with immobilized cells were stored at 4 ºC. Samples at different times were incubated in ferrous medium and these showed high cell activity even after 6 months.