RESEARCH ARTICLE


Isolation and Characterization of Amylase Enzyme Produced by Indigenous Bacteria from Sugar Factory Waste



Eli Hendrik Sanjaya1, 2, 3, 4, *, Suharti Suharti1, Mieke Alvionita1, Ivonne Telussa5, Silvia Febriana1, Hafiz Clevanota1
1 Department of Chemistry, Universitas Negeri Malang, Indonesia
2 Department of Biotechnology, Universitas Negeri Malang, Indonesia
3 Center of Advanced Materials for Renewable Energy (CAMRY), Universitas Negeri Malang, Indonesia
4 Research Center of Science and Engineering, LPPM, Universitas Negeri Malang, Indonesia
5 Department of Chemistry, Universitas Pattimura, Ambon, Indonesia


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Creative Commons License
© 2024 The Author(s). Published by Bentham Open.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Correspondence: Address correspondence to this author at the Department of Chemistry, Universitas Negeri Malang, Indonesia; E-mail: eli.hendrik.fmipa@um.ac.id


Abstract

Background

Enzymes are biocatalysts commonly used in industries. One of these enzymes is amylase. In Indonesia, most of the enzymes are still imported from overseas. To reduce the production cost, local amylase is needed which can be produced from indigenous microorganisms.

Aims

This research aims to explore isolates amylase-producing bacteria from a liquid waste of sugar factories, optimize amylase enzyme production, and identify genotypically the indigenous bacterial.

Objective

This research aims to explore isolates amylase-producing bacteria from a liquid waste of sugar factories, optimize amylase enzyme production, and identify genotypically the indigenous bacterial.

Methods

This study consists of seven stages: sample preparation, isolation of amylase-producing bacteria, crude amylase extract production, amylase activity assay, optimization of amylase enzyme production, determining the specific activity, and bacterial identification through genotyping.

Results

This research successfully identified 3 bacterial isolates (G-7, G-8, and G-12) that positively produce amylase enzymes from sugar factory waste. The optimal conditions for amylase enzyme production for all three isolates were at 37°C, pH 7.0, and during the exponential growth phase - at 24 hours for isolate G-8 with specific amylase enzyme activity of 0.198 U/mg, and at 48 hours for isolates G-7 and G-12 with specific amylase enzyme activities of 0.108 U/mg and 0.208 U/mg respectively. The 16S rRNA gene identification results showed that G-7, G-8, and G-12 belong to the species Bacillus infantis, Bacillus flexus, and Pseudomonas nitroreducens respectively.

Conclusion

The species Bacillus infantis, Bacillus flexus, and Pseudomonas nitroreducens has shown great potential for the production of amylase enzyme.

Keywords: Amylase enzyme, Bacterial isolation, Characterization, Indigenous, Optimization, Sugar factory waste.