RESEARCH ARTICLE

The Production of Aflatoxins and Ochratoxin-A by Aspergillus Strains Isolated from Rice: Under In vitro Conditions

The Open Biotechnology Journal 05 Oct 2023 RESEARCH ARTICLE DOI: 10.2174/18740707-v17-231023-2023-2

Abstract

Background:

Rice supplies a significant proportion of nutritional needs around the world. The fungal species that cause severe contamination of rice grains have created a major challenge to ensuring food safety.

Methods:

Thus, This study adopts an appropriate local method using potato dextrose agar (PDA) and thin-layer chromatography (TLC) for the production of Aflatoxins and Ochratoxin-A in Aspergillus flavus (NRRL strain 2999) and A. acrasus (NRRL strain 7431), receptively.

Results:

Promising early results suggest that an optimum protocol for the production of mycotoxin includes a temperature of 28°C for 21 d incubation. The average levels of A. flavus and A. acrasus were 625 and 482.67 μg/g, respectively, by comparing the fluorescence with the standard. As a result, a new and rapid method using PDA as a culture medium and TCL was developed to produce mycotoxins in rice from the Persian market.

Conclusion:

This study provides a novel (optimum) mechanistic approach concerning mycotoxins production from fungal species that could improve quality and ascertain its safety either in the field or in storage.

Keywords: Mycotoxin, Production, Culture media, Thin-layer chromatography, Persian market, Aflatoxin, Ochratoxin-A.
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