The Production of Aflatoxins and Ochratoxin-A by Aspergillus Strains Isolated from Rice: Under In vitro Conditions

Hasan Nazarizadeh1, *, Seyyed Mohammad Hosseini1, Javad Pourreza1
1 Department of Animal Sciences, University of Birjand, Birjand, Iran

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© 2023 Nazarizadeh et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Department of Animal Sciences, University of Birjand, Birjand, Iran; E-mail:



Rice supplies a significant proportion of nutritional needs around the world. The fungal species that cause severe contamination of rice grains have created a major challenge to ensuring food safety.


Thus, This study adopts an appropriate local method using potato dextrose agar (PDA) and thin-layer chromatography (TLC) for the production of Aflatoxins and Ochratoxin-A in Aspergillus flavus (NRRL strain 2999) and A. acrasus (NRRL strain 7431), receptively.


Promising early results suggest that an optimum protocol for the production of mycotoxin includes a temperature of 28°C for 21 d incubation. The average levels of A. flavus and A. acrasus were 625 and 482.67 μg/g, respectively, by comparing the fluorescence with the standard. As a result, a new and rapid method using PDA as a culture medium and TCL was developed to produce mycotoxins in rice from the Persian market.


This study provides a novel (optimum) mechanistic approach concerning mycotoxins production from fungal species that could improve quality and ascertain its safety either in the field or in storage.

Keywords: Mycotoxin, Production, Culture media, Thin-layer chromatography, Persian market, Aflatoxin, Ochratoxin-A.