REVIEW ARTICLE


Application of CRISPR/Cas9 Genome Editing System to Reduce the Pre- and Post-Harvest Yield Losses in Cereals



Thumadath Palayullaparambil Ajeesh Krishna1, Theivanayagam Maharajan1, Stanislaus Antony Ceasar1, *
1 Department of Biosciences, Rajagiri College of Social Sciences, Kochi-683104, Kerala, India.


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Creative Commons License
© 2022 Krishna et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Department of Biosciences, Rajagiri College of Social Sciences, Kochi-683104, Kerala, India; Email: antony_sm2003@yahoo.co.in


Abstract

Cereals are an important source of food for millions of people across low-middle-income countries. Cereals are considered a staple food for poor people. The majority of the people are depending upon agricultural occupation. Agriculture provides a primary source of income for many farmers in low-middle-income countries. The pre- and post-harvest loss of crop yield affects farmers and is a major problem in achieving food security. Biotic and abiotic factors cause pre- and post-harvest loss of crop yield worldwide. It significantly affects the economic status of farmers as well as low-middle-income countries. Many advanced technologies are available for resolving the pre- and post-harvest loss of crop yield. The past few decades have seen remarkable progress in crop improvement. Especially high-throughput genome sequencing approaches contributed to advancement in the crop improvement. Genome-editing has also been considered a key tool for crop improvement. The clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system has become a potent genome editing system for modifying key traits in cereal crops. CRISPR/Cas9 system offers new opportunities for addressing pre-and post-harvest constraints affecting cereal grain production and storage. In this review, we discuss the application of the CRISPR/Cas9 genome editing system to reduce pre-and post-harvest yield loss in cereal crops. It may promote the economic status of farmers and reduce food demand in the coming decades.

Keywords: Cereals, Crop improvement, CRISPR/Cas9, Food security, Pre-and post-harvest, Yield loss.