RESEARCH ARTICLE


Molecular Characterization of Fusarium Solani Degrades a Mixture of Low and High Molecular Weight Polycyclic Aromatic Hydrocarbons



Abd El-Latif Hesham1, *, Elsayed A. Mohamed1, Asmaa M.M. Mawad2, Ameer Elfarash1, Bahaa S. Abd El-Fattah1, Mahmoud A. El-Rawy1
1 Genetics Department, Faculty of Agriculture, Assiut University, Assiut, Egypt
2 Botany and Microbiology, Faculty of Science, Assiut University, 71516, Assiut, Egypt


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© 2017 Hesham et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Genetics Department, Faculty of Agriculture, Assiut University, Assiut, Egypt; Tel: +2-01012115256; E-mail: hesham_egypt5@aun.edu.eg


Abstract

Objectives:

This study evaluates the ability of a non-white rot fungus strain, HESHAM-1, to degrade a mixture of low (naphthalene and phenanthrene) and high (chrysene and benzo(a)pyrene) molecular weight polycyclic aromatic hydrocarbons (LMW and HMW PAHs).

Methods:

Strain HESHAM-1 was isolated from oil polluted soil by enrichment method using phenanthrene as the sole source of carbon and energy. The strain showed the ability to tolerate and degrade a mixture of both low and high molecular weight PAHs. In the presences of LMW-PAHs (naphthalene and phenanthrene) as co-substrate, chrysene and benzo(a)pyrene (HMW-PAHs) were, respectively degraded by the fungus strain HESHAM-1 which was confirmed by GC-MS analyses.

Results:

The degradation rate was found as 84.82% for naphthalene, 40.09% for phenanthrene, 57.84% for chrysene and 71.06% for benzo(a)pyrene at the end of 10 days. This is the first report describing the biodegradation of a mixture of four PAH compounds by non-white rot fungus strain HESHAM-1 isolated from Egyptian oil-polluted soil. The fungus strain HESHAM-1 was identified by morphological characteristics and molecular genetics technique based on PCR amplification and sequencing of the internal transcribed spacers (ITSs) of the rDNA region and intervening 5.8S rRNA gene. Blast result and phylogenetic analysis of gene sequencing suggested that strain HESHAM-1 was closely related to Fusarium solani with 100% sequence identity.

Conclusion:

The present study clearly demonstrates that, strain HESHAM-1 could be used to remove the crude oil from the environment.

Keywords: Non-white-rot fungus, Biodegradation, PAHs mixture, 5.8S rRNA gene, Phylogenetic analysis, Internal transcribed spacers.