RESEARCH ARTICLE


Preliminary Test of Candidate Rapid Diagnostic Test for the Detection of 38 kDa Mycobacterium Tuberculosis Antigen in Saliva



Tri Yudani Mardining Raras1, *, Nabila Rahmadani2, Maimun Zulhaidah Arthamin3, Muhammad Rizki1, 4, 5
1 Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
2 Magister Program of Biomedical Science, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
3 Department of Clinical Pathology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
4 West Nusa Tenggara Hepatitis Laboratory, Mataram, Indonesia
5 Faculty of Medicine, Universitas Mataram, Mataram, Indonesia


Article Metrics

CrossRef Citations:
0
Total Statistics:

Full-Text HTML Views: 457
Abstract HTML Views: 296
PDF Downloads: 244
ePub Downloads: 207
Total Views/Downloads: 1204
Unique Statistics:

Full-Text HTML Views: 304
Abstract HTML Views: 239
PDF Downloads: 214
ePub Downloads: 183
Total Views/Downloads: 940



Creative Commons License
© 2024 The Author(s). Published by Bentham Open.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia; E-mail: daniraras@ub.ac.id


Abstract

Background and Objectives

Identifying tuberculosis (TB) in pediatric cases is a major challenge in developing countries, as children have problems with expelling sputum, making specific diagnostics crucial. The objective of the study was to develop a rapid test using polyclonal antibodies to detect antigen 38kDa from Mycobacterium tuberculosis in the saliva of TB patients.

Materials and Methods

The recombinant protein Ag38 was purified using the Ni-NTA purification kit. Polyclonal antibodies were generated in BALB-c mice using 50 µg/ml of purified Ag38 recombinant protein. A Lateral Flow Assay (LFA) was constructed, employing 5 mg/mL colloidal gold-labelled polyclonal antibody anti-Ag38 in the test line to capture the conjugates, while goat anti-mouse IgG was used in the control line. The LFA was tested in 5 TB patients and 7 healthy person served as negative control .

Results

The recombinant protein achieved 95% purity. The rapid test kit, with a detection limit of 5.3 µg/mL, successfully identified Ag38 protein in TB patient saliva (positive control) and not in healthy human serum (negative control). While reproducibility was confirmed for TB patients, results were inconsistent for healthy individuals.

Conclusion

The Lateral Flow Assay using polyclonal antibody Ag38 displays promise in detecting M tuberculosis antigen in the saliva of TB patients. Further validation with more TB patient saliva samples is needed to determine LFA sensitivity and specificity.

Keywords: Antigen Ag38, Mycobacterium tuberculosis, Saliva, Lateral flow assay, Polyclonal antibody, Rapid test.