RESEARCH ARTICLE
Isolation and Characterization of Polyethylene and Polyethylene Terephthalate-degrading Bacteria from Jakarta Bay, Indonesia
Aqil Azizi1, *, Sirin Fairus1, Deffi Ayu Puspito Sari1
Article Information
Identifiers and Pagination:
Year: 2024Volume: 18
E-location ID: e18740707280343
Publisher ID: e18740707280343
DOI: 10.2174/0118740707280343231208102253
Article History:
Received Date: 05/09/2023Revision Received Date: 29/10/2023
Acceptance Date: 21/11/2023
Electronic publication date: 06/02/2024
Collection year: 2024
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Introduction
Plastic is resistant to natural breakdown because of its intricate structure, which features long and repeated molecular chains. As a result, a variety of plastic waste, mostly made of polyethylene (PE) and polyethylene terephthalate (PET), accumulates in Jakarta Bay. The use of microorganisms to degrade plastic trash has emerged as a highly promising bioremediation strategy.
Methods
The goal of this research is to find microorganisms capable of digesting plastic in the samples of seawater and sediment obtained from Muara Angke Jakarta Bay. The bacteria were grown on Zobell Marine Agar (ZMA) that had been treated with 2% polyethylene glycol (PEG). The bacteria were then selected based on their capacity to degrade PE and PET microplastics in a liquid medium. The ability to degrade was determined by measuring the optical density (OD) at 600 nm and the decrease in plastic dry weight after a 14-day incubation period.
Results
Seven bacterial isolates capable of decomposing PE and PET were found during the isolation and screening methods. The WJ1 outperformed other isolates in the degradation of PE and PET, with degradation rates of 4.5% and 6.5%, respectively.
Conclusion
According to 16S rRNA analysis, five bacterial species have been identified as playing a part in the process of plastic degradation: Vibrio alginolyticus, Pseudoalteromonas caenipelagi, Microbulbifer pacificus, Pseudomonas marincola, and Bacillus subtilis. The ability of these bacteria to biodegrade plastics represents an opportunity to effectively remove persistent pollutants from the environment.
