Indirect Labeling of Antibodies as a Universal Approach to Increase Sensitivity of Lateral Flow Tests: A Case Study for Mycotoxins Detection



Alexandr E. Urusov1, Alina V. Petrakova1, Anatoly V. Zherdev1, Elena A. Zvereva1, *
1 Research Centre of Biotechnology of the Russian Academy of Sciences, Bach Institute of Biochemistry, Moscow, Russia

Abstract

Objectives:

The study aimed at increasing the sensitivity of immunochromatographic tests for the control of toxic contaminants (on the examples of aflatoxin B1 and T-2 toxin) in agricultural products.

Methods:

For reliable immunochromatographic detection of low concentrations of analytes, a replacement of the (specific antibodies – gold nanoparticle) conjugate by a combination of native specific antibodies and anti-species antibodies conjugated with gold nanoparticles was proposed. Different variants of test systems based on the principle of indirect labeling were realized and compared.

Results:

Immunochromatographic assays with indirect labeling for aflatoxin B1 and T-2 toxin were implemented experimentally. A reduction in the detection limit by one to two orders of magnitude was demonstrated.

Conclusion:

The presented results confirm that indirect labeling of specific antibodies overcomes the limitations of the competitive immunochromatographic analysis and can be used to detect analytes of different chemical nature.

Keywords: Mycotoxins, Food safety, Immunochromatography, Gold nanoparticles, Assay sensitivity, Lateral flow tests.


Abstract Information


Identifiers and Pagination:

Year: 2019
Volume: 13
Publisher Item Identifier: EA-TOBIOTJ-2019-HT1-951-1

Article History:

Received Date: 05/04/2019
Revision Received Date: 15/07/2019
Acceptance Date: 27/09/2019
Electronic publication date: 09/10/2019
Collection year: 2019

© 2019 Urusov et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


* Research Centre of Biotechnology of the Russian Academy of Sciences, Bach Institute of Biochemistry, Moscow, Russia; Tel: +7 495 954 28 04;
E-mail: zverevaea@yandex.ru