Indirect Labeling of Antibodies as a Universal Approach to Increase Sensitivity of Lateral Flow Tests: A Case Study for Mycotoxins Detection

Alexandr E. Urusov1, Alina V. Petrakova1, Anatoly V. Zherdev1, Elena A. Zvereva1, *
1 Research Centre of Biotechnology of the Russian Academy of Sciences, Bach Institute of Biochemistry, Moscow, Russia



The study aimed at increasing the sensitivity of immunochromatographic tests for the control of toxic contaminants (on the examples of aflatoxin B1 and T-2 toxin) in agricultural products.


For reliable immunochromatographic detection of low concentrations of analytes, a replacement of the (specific antibodies – gold nanoparticle) conjugate by a combination of native specific antibodies and anti-species antibodies conjugated with gold nanoparticles was proposed. Different variants of test systems based on the principle of indirect labeling were realized and compared.


Immunochromatographic assays with indirect labeling for aflatoxin B1 and T-2 toxin were implemented experimentally. A reduction in the detection limit by one to two orders of magnitude was demonstrated.


The presented results confirm that indirect labeling of specific antibodies overcomes the limitations of the competitive immunochromatographic analysis and can be used to detect analytes of different chemical nature.

Keywords: Mycotoxins, Food safety, Immunochromatography, Gold nanoparticles, Assay sensitivity, Lateral flow tests.

Abstract Information

Identifiers and Pagination:

Year: 2019
Volume: 13
Publisher Item Identifier: EA-TOBIOTJ-2019-HT1-951-1

Article History:

Received Date: 05/04/2019
Revision Received Date: 15/07/2019
Acceptance Date: 27/09/2019
Electronic publication date: 09/10/2019
Collection year: 2019

© 2019 Urusov et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: ( This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Research Centre of Biotechnology of the Russian Academy of Sciences, Bach Institute of Biochemistry, Moscow, Russia; Tel: +7 495 954 28 04;